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| | FRET Assay for LRH-1 |
| Description: | The screen utilizes a ligand mediated co-factor interaction between purified bacterial expressed ligand binding domain of human LRH1 and a TIF2-derived peptide. Detection of the associated complex was measured by time resolved fluorescence energy transfer (TR-FRET). The EC50s of the test compound was estimated from a plot using the ratio of fluorescence values collected at 671 nm to fluorescence values collected 618 nm versus concentration of test compound added. Test compounds (agonists) that increased the affinity of the receptors for the peptide yielded an increase in fluorescent signal. |
| Affinity data for this assay | |
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