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Assay Method Information

Assay Name:  FAAH Inhibition Assay
Description:  The inhibition assays were performed by incubating enzyme, 14C-labeled oleamide in reaction buffer at room temperature in the presence of three different concentrations of inhibitor. The 14C-labeled oleamide (substrate) and oleic acid (product) were extracted and analyzed by TLC. The Ki of the inhibitor was calculated using a Dixon plot. Lineweaver-Burk analysis was performed, confirming competitive, reversible inhibition.
Affinity data for this assay
 

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Last update November 1, 2007
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