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| | Evaluation of Inhibitors with Recombinant LC/A |
| Description: | Recombinant BoNT LC/A activity was measured in black 96-well microtiter plates by use of a Molecular Devices (Sunnyvale, CA) SpectraMax GeminiEM plate reader. Fluorimeter parameters consisted of excitation@490 nm (slit width =2 nm), an emission@532 nm (slit width =2 nm), and a cut-off filter at 495 nm. Initial rates were measured from the linear region of each assay. IC50 values were determined by using equation that includes initial rates in the presence/absence of inhibitor. |
| Affinity data for this assay | |
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