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Assay Method Information |
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| | Epoxide Hydrolase Assay |
| Description: | Recombinant human LTA4H was incubated with various concentrations of test compound for 10 min at room temperature in assay buffer, and the substrate, LTA4, was added. After 10-30 min at room temperature, the assay was terminated, and the amount of LTB4 produced was assayed by enzyme immunoassay (EIA). The concentration of compound that was required for half-maximal inhibition of recombinant enzyme activity (IC50) was calculated by nonlinear regression using Graphpad Prism 4.0, one site binding competition. |
| Affinity data for this assay | |
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