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| | Enzyme Inhibition Assay |
| Description: | The inhibitory activity of the test compounds against human recombinant glycogen phosphorylase a (GPa) was monitored using 96-well microplate format. The glucose-1-phosphate production from glycogen monitored by a multienzyme coupled assay. The change in optical density due to inorganic phosphate formation was measured at 620nM in a Labsystems iEMS Reader MF. The IC50 values were estimated by fitting the inhibition data to a dose dependent curve using a logistic derivative equation. |
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