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Assay Method Information

Assay Name:  Akt1 Kinase Assay
Description:  A fluorescence polarization IMAP type assay is used. After a 90 min incubation of reaction mixtures containing enzyme, substrate, and inhibitor, IMAP beads are added and plates are read (lamp filter: 544 nm, emission filter: 615 nm).
Affinity data for this assay
 

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Last update November 1, 2007
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