Assay Method Information |
|
| Plk in Vitro Assay |
Description: | An IMAP fluorescence polarization-based assay format (Molecular Devices) was used to determine the ability of compounds to inhibit the phosphorylation of a fluorophore tagged substrate peptide. Kinase activity was measured by change in fluorescence polarization units detected using an LJL Analyst (LJL Biosystems). IC50 values were calculated using a four parameter fit and GraphPad Prism software. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |