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| | ROCK Kinase Inhibition Assay |
| Description: | The assay of Rock-1 activity involved incubation with peptide substrate and ATP33, and the incorporation of P33 into the peptide was quantified by Scintillation Proximity Assay (SPA - Amersham Pharmacia). For IC50 determination, compounds were assayed over an eleven point dilution range with a concentration in the assay of 50 uM to 0.8 nM. IC50 values were calculated by bespoke curve fitting software. |
| Affinity data for this assay | |
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