Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | InhA Enzyme Inhibition Assay |
| Description: | The assay measured the NADH-dependent catalysis of an octenoyl-CoA substrate as a decrease in 340 nm absorbance resulting from conversion of NADH to NAD. The absorbance OD readings were taken on a Molecular Device SPECTRAmax PLUS 384 microreader. The IC50 values were determined using at least eight concentrations, with each concentration assayed in triplicate under saturating substrate conditions. The values were calculated from plots of enzyme activity versus the log of the inhibitor concentration using the GraFit IC50 four-parameter fit software. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||