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| | RAF Kinase Inhibition Assay |
| Description: | To measure compound inhibitor effects on the ability of Raf enzymes to phosphorylate the Mek substrate, a capture ELISA utilizing streptavidin-coated plates was developed. Signal detection is by Time Resolved Flouresence utilizing a Europium labeled goat anti-rabbit secondary antibody, subsequently followed by fluoremetry with the Victor reader (Wallac-PerkinElmer). |
| Affinity data for this assay | |
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