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Assay Method Information

Assay Name:  Receptor Binding Assay
Description:  The membranes prepared from HEK cells transfected with adenosine receptors were used in binding assays. Nonspecific binding was determined in the presence of excess of DPCPX or CGS15943 for the A1 and A2A membranes, respectively. Bound and free ligands were separated by rapid vacuum filtration, and the bound radioligand was determined by scintillation counting. Binding data were analyzed by nonlinear least-squares curve-fitting algorithms using GraphPad Prism. Ki values were calculated from IC50 values using the Cheng-Prusoff equation.
Affinity data for this assay
 

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Last update November 1, 2007
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