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Assay Method Information

Assay Name:  Aurora B Kinase Inhibition Assay
Description:  In vitro kinase assay using recombinant Aurora B bound to INCENP was incubated at room temperature with substrate, and test compounds in the presence of 15 uM ATP/ [gamma-33P] ATP. 33P incorporation was measured with a Beta Counter. IC50 values were calculated using ORIGIN Scientific Graphing Analyses.
Affinity data for this assay
 

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Last update November 1, 2007
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