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| | LANCE Assay |
| Description: | Analysis of pharmacological properties of compounds was performed using the LANCE cAMP kit (Perkin Elmer). HEK293 cells stably expressing the hMC4R were treated with PAM compounds at doses indicated, then incubated with an Alexa Fluor 647-labeled antibody in stimulation buffer according to manufacturer's instructions for 30 min at RT. Next, α-MSH was added (at an EC20 dose or at doses indicated for concentration-response curves), and plates were incubated for 15 minutes at RT, followed by the addition of 10 μL of detection mix. Plates were then incubated for 30 minutes at RT and read on a Viewlux (Perkin Elmer) using 618 and 671 nm emission filters. IBMX was not included in the assay. |
| Affinity data for this assay | |
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