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Assay Method Information |
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| | FAAH Carbon Filtration Assay |
| Description: | [3H]anandamide was incubated with membranes to produce radiolabeled ethanolamine and unlabeled arachidonic acid. Charcoal selectively binds anandamide and arachidonic acid, but not ethanolamine. Uncleaved [3H] anandamide and the unlabeled arachidonic acid are absorbed by the charcoal. In contrast, the labeled [3H] ethanolamine flows through the charcoal minicolumns. The plates can then be read on a Hewlett-Packard TopCount. |
| Affinity data for this assay | |
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