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| | IGF-1R Inhibition Assay |
| Description: | Assays are performed in 384-well microtiter plates in reaction buffer containing biotinylated substrate, ATP, and purified activated GST-IGF-1R in the presence of test compounds. After incubation, Streptavidin-APC and Europium conjugated phospho-tyrosine antibodies (Perkin-Elmer) are added for signal detection. The signal is read on PerkinElmer Viewlux microplate imager or Wallac Victor fluorometer. |
| Affinity data for this assay | |
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