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| | COT HTRF Assay |
| Description: | The COT direct HTRF assay was carried out using biotin-MEK1 as a substrate. The reaction was carried out in black 96 half-well plates. At designated time points, EDTA was added to quench the reaction. Detection reagents were then added to each well to make anti-phospho-MEK1-Eu antibody and SAXL. The plates were incubated, and then read using the RUBYstar HTRF reader. |
| Affinity data for this assay | |
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