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| | Cell-Based Assay for Amyloid-beta Accumulation |
| Description: | A whole-cell gamma-secretase inhibition assay using SHSY5Y neuroblastoma cells in which human gamma-secretase catalyzes the breakdown of the overexpressed exogenous substrate A4CTF construct. Amyloid-beta peptides secreted into the cell culture medium were quantified using the homogeneous time-resolved fluorescence (HTRF) assay by comparison against synthetic peptide standards. |
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