Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | Spectral Titration of TCCYP51 |
| Description: | As a cysteine-coordinated hemoprotein, sterol 14-alpha-demethylase responds spectrally to any perturbations in the area surrounding the heme iron. These spectral responses can be used to estimate the apparent dissociation constants (Kd) of the enzyme/ligand complexes. The titration experiments were carried out at in a cuvette containing TCCYP51 in the wavelength range 350-500 nm using a Shimadzu UV-2401PC spectrophotometer. The tested compounds were added in 1 ul aliquots to the test cuvette until the maximum in spectral response was reached. Equal volumes of DMSO were added to the reference cuvette. The apparent Kd values were determined from the equilibrium titration curves by plotting absorbance changes against the concentration of free ligand and fitting the data to a rectangular hyperbola using SigmaPlot Statistics. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||