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Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  Aurora kinase was assayed in ELISA format using a GST fusion of the N-terminus of Histone H3 as substrate. Plates were coated with substrate, and then blocked with I-block (Tropix) in PBS. After kinase reaction, product was detected by incubation with antiphosphohistone H3 (Ser10) 6G3 mouse monoclonal antibody and sheep-antimouse HRP conjugate, followed by washing and addition of TMB substrate. Plates were read at 450 nm.
Affinity data for this assay
 

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Last update November 1, 2007
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