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Assay Method Information |
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| | Retinoid Competition Binding Assay |
| Description: | Recombinant RAR protein expressed in E. coli was used in the direct binding assay. The apparent dissociation constants (Kd) were determined by the charcoal absorption method. After incubation, unbound [3H]RA was removed by addition of equivalent-sized dextran-treated charcoal. The scintillation cocktail (Beckman) was added 0.5 mL of the supernatant, and it was then counted in an LS6500 scintillation counter (Beckman) using the auto-dpm program. |
| Affinity data for this assay | |
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