Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | In Vitro Protein Kinase Assay |
| Description: | NSC 109555 was diluted in water. All other drugs were dissolved in DMSO, in which case the final DMSO concentration in reactions was 10%, and the controls were performed under comparable conditions. Recombinant Chk2 was incubated with either histone H1 (or GST-Cdc25C) in reaction buffer, and incubated at 30 deg C for indicated times. For the drug inhibition experiments, samples were coincubated with drug during the reactions. Reactions were stopped by adding sample buffer, and samples were boiled for 5 min. Reaction products were separated by 4-20% SDS-PAGE. Chk2 protein kinase activity, measured as 32P incorporation into Chk2, histone H1 (or GST-Cdc25C), was determined using a PhosphorImager (GE Healthcare, Chalfont St. Giles, Buckinghamshire, UK). Densitometry was performed using Image-Quant. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||