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Assay Method Information

Assay Name:  Counterscreen for inhibitors of tRNA 2'-phosphotransferase (TPT1): fluorescence polarization-based biochemical high throughput dose response assay to identify inhibitors of RNAse T1.
Description:  Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute (TSRI) Assay Provider: Heather Harding, New York University School of Medicine Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: 1-R03-DA026554-01 Grant Proposal PI: Heather Harding, New York University School of Medicine External Assay ID: RNASETI _INH_FP_1536_3XIC50 Name: Counterscreen for inhibitors of tRNA 2'-phosphotransferase (TPT1): fluorescence polarization-based biochemical high throughput dose response assay to identify inhibitors of RNAse T1. Description: The process of transcription converts the DNA sequences found in genes into mRNA (1, 2). This process is coupled to the subsequent removal of mRNA introns by splicing, which additionally serves to increase proteome complexity (3, 4). Intron splicing also occurs for transfer RNA (tRNA), which functions in the delivery of amino acid
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Last update November 1, 2007
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