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Assay Method Information

Assay Name:  Counterscreen for inhibitors of TLR9-MyD88 binding: fluorescence-based cell-based high throughput dose response assay to identify non-selective inhibitors of the beta-lactamase enzyme (BLA)
Description:  Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Affiliation: The Scripps Research Institute, TSRI Assay Provider: Peter S. Tobias, The Scripps Research Institute (TSRI) Network: Molecular Library Probe Production Centers Network (MLPCN) Grant Proposal Number: RO1 AI078288 Grant Proposal PI: Peter S. Tobias, The Scripps Research Institute (TSRI) External Assay ID: BLA_INH_BLA_1536_3XIC50 DCSRUN Name: Counterscreen for inhibitors of TLR9-MyD88 binding: fluorescence-based cell-based high throughput dose response assay to identify non-selective inhibitors of the beta-lactamase enzyme (BLA). Description: Toll-like receptors (TLRs) are involved in initiating inflammatory responses to a number of components of pathogens as well as markers of sterile injury. As such they initiate homeostatic responses but also pathologic responses. Our focus on TLR9 is based on its role in autoimmune disease. Auto-antibodies to DNA, RNA, or both are characteri
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