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| | In Vitro Kinase Assay |
| Description: | The IC50 for the different compounds was determined with Invitrogen Z'-LYTE kinase assay kit Ser/Thr 1 peptide PV3174 by following the manufacturer's instructions. To determine the IC50 of receptor tyrosine kinase, an assay based on the ELISA principle with kinases from Biomol (N-terminally fused to GST), POD-coupled antibody from Calbiochem (PY20)) and BM chem chemiluminescence ELISA substrate from Roche was performed. |
| Affinity data for this assay | |
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