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Assay Method Information |
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| | ChEMBL_1920945 (CHEMBL4423790) |
| Description: | Inhibition of human DHFR expressed in Escherichia coli BL21 competent cells using DHF as substrate preincubated for 15 mins followed by substrate and NADPH addition and measured after 60 mins by resazurin dye based diaphorase-coupled assay |
| Affinity data for this assay | |
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