Assay Method Information |
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| ChEMBL_2200644 (CHEMBL5113160) |
Description: | Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21(DE3) assessed as reduction in all-trans isomers of LTB4 and 5-HETE formation using arachidonic acid as substrate preincubated with enzyme for 15 mins followed by substrate addition and measured after 10 mins by RP-HPLC analysis |
Affinity data for this assay | |
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