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Assay Method Information |
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| | ChEMBL_1629346 (CHEMBL3871972) |
| Description: | Inhibition of N-terminal His6-tagged DNMT3A (623 to 908 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) coexpressing DNMT3L (160 to 386 C-terminal residues) (unknown origin) assessed as reduction in oligonucleosome methylation preincubated for 15 mins followed by addition of [3H]-SAM measured after 4 hrs by liquid scintillation counting method |
| Affinity data for this assay | |
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