Assay Method Information |
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| Enzyme Inhibition Assay |
Description: | The IC50 value is defined as the concentration of an inhibitor with caused 50% reduction of the enzyme activity under specific condition. The principle of these assay with synthetic 2-naphthylamide substrates is based on the absorbance at 530nm of a chromophoric complex formed by the reaction product 2-naphthylamine with Fast Blue B salt. |
Affinity data for this assay | |
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