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Assay Method Information |
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| | Enzymatic Assay |
| Description: | Compounds were tested for their inhibition of the addition of L-Ala, D-Glu, L-Lys or D-Ala-D-Ala to nucleotide precursors catalyzed by MurC from Escherichia coli, MurD from E. coli, MurE from Staphylococcus aureus, and MurF from E coli. The detection of the orthophosphate generated during the reaction was based on the colorimetric Malachite green method. |
| Affinity data for this assay | |
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