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Assay Method Information |
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| | Nuclear Magnetic Resonance (NMR) Assay |
| Description: | NMR spectra were acquired on Bruker Avance 600 MHz spectrometers at 298 K using a 5 mm triple-resonance HCN cryoprobe. Ligand binding was detected using WaterLOGSY and T1p-filtered 1D experiments in combination with 2D 1H-15N TROSY-HSQC experiments to provide binding-site and kd information. |
| Affinity data for this assay | |
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