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Assay Method Information |
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| | Fluoresence-based Assay |
| Description: | The initial screen was performed in a 96-well plate using the fluoresence-based assay previously described. An enzyme-coupled absorbance-based assay developed in house was used to determine the inhibitor-enzyme kinetic parameters of Mtb-AnPRT, including ki values for the inhibitors. |
| Affinity data for this assay | |
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