Assay Method Information |
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| Homogenous Time-Resolved Fluorescence (HTRF) Assay |
Description: | MARK3 activity was assayed in vitro using a Cdc25C biotinylated peptide substrate (Cell Signalling Technologies). The phosphopeptide product was quantitated using a Homogenous Time-Resolved Fluorescence (HTRF) assay system (Park et al., 1999, Anal. Biochem. 269:94-104). |
Affinity data for this assay | |
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