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Assay Method Information

Assay Name:  Homogenous Time-Resolved Fluorescence (HTRF) Assay
Description:  MARK3 activity was assayed in vitro using a Cdc25C biotinylated peptide substrate (Cell Signalling Technologies). The phosphopeptide product was quantitated using a Homogenous Time-Resolved Fluorescence (HTRF) assay system (Park et al., 1999, Anal. Biochem. 269:94-104).
Affinity data for this assay
 

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Last update November 1, 2007
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