new BindingDB logo
myBDB logout

Assay Method Information

Assay Name:  Kinase Inhibition Assay
Description:  The EGF-R kinase autophosphorylation activity was measured by DELFIA/time-resolved fluorometry with excitation at 340 nm and emission at 615 nm. Positive and negative controls were included in each plate by incubation of enzyme with or without ATP-MgCl2. The percentage of autophosphorylation inhibition by the compound was calculated using the equation: 100 - [(test - negative control)/(positive control - negativecontrol)]. The IC50 was obtained from curves of percentage inhibition.
Affinity data for this assay
 

If you find an error in this entry please send us an E-mail
   
    

Home

|

Search

|

Deposit

|

SiteMap

|

About us

|

Email us

|

Info

 
Last update November 1, 2007
©2000 BindingDB. All rights reserved.