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| | Counterscreen for inhibitors of phospholipase C isozymes (PLC-B3): Fluorescence-based biochemical high throughput dose response assay to identify inhibitors of phospholipase C isozymes (PLC-gamma1) |
| Description: | Source (MLPCN Center Name): The Scripps Research Institute Molecular Screening Center (SRIMSC) Center Affiliation: The Scripps Research Institute Assay Provider: Qisheng Zhang, University of North Carolina at Chapel Hill Network: Molecular Libraries Probe Production Centers Network (MLPCN) Grant Proposal Number: R01GM098894 Grant Proposal PI: Qisheng Zhang, University of North Carolina at Chapel Hill External Assay ID: PLCG1_INH_QFRET_1536_3XIC50 DCSRUN for PLCB3 INH Name: Counterscreen for inhibitors of phospholipase C isozymes (PLC-B3): Fluorescence-based biochemical high throughput dose response assay to identify inhibitors of phospholipase C isozymes (PLC-gamma1). Description: Extracellular stimuli including hormones, growth factors, and neurotransmitters promote activation of phospholipase C (PLC) isozymes and cleavage of the membrane lipid phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2) into the classical second messengers, diacylglycerol and inositol 1,4,5- trisphosph |
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