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Assay Method Information

Assay Name:  mTOR Kinase Activity Assay
Description:  The mTOR kinase TR-FRET assay utilizes a physiologically relevant protein substrate for mTOR (4E-BP1, labeled with an acceptor fluorophore (Green Fluorescent Protein) and paired with a corresponding Tb-labeled phospho-specific antibody. The assay itself can be divided into two phases: the reaction phase and the detection phase.
Affinity data for this assay
 

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Last update November 1, 2007
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