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Assay Method Information |
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| | TgENR Inhibition Assay |
| Description: | The reaction mixture (100 µL) contained 100 µM crotonyl-CoA, 1 µL DMSO (or compounds dissolved in DMSO), 5 nM TgENR, 100 mM sodium/potassium phosphate (pH 7.5), 150 mM NaCl, and 100 µM NADH. Each compound was measured in duplicate at a final concentration of 1 µM. |
| Affinity data for this assay | |
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