Assay Method Information |
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| Inhibition of DDP IV In Vitro |
Description: | The reaction contained 10 uM of Ala-Pro-AMC, 50 pM of enzyme DDP-4, different concentrations of test compounds, and assay buffer (100 mM HEPES, pH 7.5, 0.1 mg/mL BSA) in a total reaction volume of 50 uL. |
Affinity data for this assay | |
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