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Assay Method Information |
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| | Spectral Binding Assay |
| Description: | The buffer contained 50 mM HEPES (pH 7.5), 10% glycerol, and 100 mM NaCl, containing 1 mM imidazole. The spectral shift was monitored for a few minutes in room temperature after each addition of increasing amounts of inhibitors to 5-6 μM protein. |
| Affinity data for this assay | |
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