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Assay Method Information |
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| | Intrinsic-Fluorescence Titration |
| Description: | Purified ThiT was diluted in buffer E to a concentration of ~50 nM in a final volume of 800 µL. The substrates were added in 1 µL steps. Using an excitation wavelength of 280 nm, emission was measured at 350 nm and 25 °C. After each addition of substrate, 10 s were allowed for equilibration and mixing, and the signals were averaged over 15 s. |
| Affinity data for this assay | |
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