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Assay Method Information |
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| | Amplex Red MAO Assay |
| Description: | Study medium contained 0.1 mL of sodium phosphate buffer (0.05 M, pH 7.4), various concentrations of the newly synthesized compounds or reference compounds, and adequate amounts of recombinant hMAO-A or hMAO-B. This mixture was incubated for 15 min at 37 °C in a 96-well microplate, placed in the dark fluorimeter chamber. After this incubation period, the reaction was started by adding 200 µM Amplex Red reagent, 1 U/mL horseradish peroxidase (HRP), and p-tyramine (concentration range 0.05-1.00 mm). |
| Affinity data for this assay | |
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