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Assay Method Information |
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| | Fluorescence Titration Assay |
| Description: | To assess direct binding of inhibitors to oxidized NQO2 (NQO2ox), fluorescence quenching of FAD was monitored with an excitation wavelength of 350 nm and an emission wavelength of 430 nm. NQO2 (775 or 38.75 nM) was titrated with the indicated concentrations of TBB, TBBz, and DMAT. |
| Affinity data for this assay | |
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