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Assay Method Information |
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| | Kinase Assay |
| Description: | The test methods for GSK-3β, PKCE, JAK2, BRAF, IKK2 and Drak2 inhibitory activities were reported in Molecules; 18:5498-5516. The PKA kinase assay was carried out with the Invitrogen Z -LYTETM Kinase Assay kit (Ser/Thr 1 Peptide substrate), with a final enzyme concentration of 4 nM. The AKT1 kinase assay was carried out with the Invitrogen Z -LYTETM Kinase Assay kit (Ser/Thr 6 Peptide substrate), with a final enzyme concentration of 4 nM. CDK2/CycA2 kinase assay was carried out with the Invitrogen Z -LYTETM Kinase Assay kit (Ser/Thr 12 Peptide substrate), with a final enzyme concentration of 2 nM. All reactions were carried out in triplicate. |
| Affinity data for this assay | |
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