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Assay Method Information |
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| | Colorimetric Assay |
| Description: | Briefly, the tested compounds were solubilized in DMSO and serially diluted into concentrations for the inhibitory test. The assays were carried out in a final volume of 100 µL containing 50 mmol/L MOPS, pH 6.5, 2 mmol/L pNPP, 21 nmol/L GST-PTP1B or GST-TCPTP, and 2% DMSO, and the catalysis of pNPP was continuously monitored on a SpectraMax 340 microplate reader at 405 nm for 3 min at 30 °C |
| Affinity data for this assay | |
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