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| | Enzymatic Activity Assay |
| Description: | The mushroom tyrosinase inhibition activity of hydroxy naphthylchalcone compounds was measured using L-DOPA as substrate. The activity assay used 3 ml of reaction medium containing 0.5 mM L-DOPA in 50 mM Na2HPO4-NaH2PO4 buffer (pH 6.8). The final concentrations of tyrosinase was 3.33 mg/ml. The substrate reaction progress curve was analyzed to obtain the reaction rate constants. The reaction was carried out at 30 °C and pH 6.8. |
| Affinity data for this assay | |
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