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Assay Method Information

Assay Name:  DHFR Inhibition Assay
Description:  The recombinant human (rh) DHFR was purchased from Sigma-Aldrich (Shanghai, China). All rhDHFR enzymes were assayed spectrophotometrically in a solution containing 0.1 mM dihydrofolate, 0.3 mM NADPH and 100 mM KCl at pH 7.5 and 32 C. The reaction was initiated with an amount of enzyme yielding a change in O.D. at 340 nm measured by microplate spectrophotometer.
Affinity data for this assay
 

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Last update November 1, 2007
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