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Assay Method Information |
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| | Kinase Inhibition Assay |
| Description: | For kinase inhibition assays, the final concentrations of the enzymes were 15, 14.5, 14.5, and 8.8 nM for CLK1, CLK2, CLK3, and Dyrk1A (Invitrogen, catalog no. PV3785), respectively. The compound concentrations ranged from 15503 to 121 nM and from 1550 to 12 nM. The RS domainderived peptide (GRSRSRSRSR) (Anaspec, catalog no. 61722) for CLK1 and CLK3, Dyrktide (RRRFRPASPLRGPPK) (Anaspec, catalog no. 62698) for Dyrk1A, and S6K peptide for CLK2 were used as substrates at a concentration of 5 μM. |
| Affinity data for this assay | |
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