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Assay Method Information

Assay Name:  Inhibition Kinetics
Description:  IC50 values for the inhibition of wildtype and mutant forms of ypFabV were performed by adding varying concentrations of inhibitor dissolved in dimethylsulfoxide (DMSO) to reaction mixtures containing 250 μM NADH, 30 μM ddCoA, and 15 nM enzyme. The total amount of DMSO added was kept constant (final DMSO concentration of 2%).
Affinity data for this assay
 

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Last update November 1, 2007
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