Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | UV-Visible Titration Assay |
| Description: | All UV-visible spectra were measured on a Varian Cary 50 spectrometer with temperature control from a Peltier cooler. The holo-enzyme was titrated in 0.1M potassium phosphate buffer starting at pH 6.8, 25 °C, and finishing at pH 5.1 or 10.2. Each titration was conducted in a 2-ml cuvette with the protein at ~8 uM heme-containing monomer, where the heme concentration was determined via the pyridine hemochrome assay. Solutions were continuously stirred, and the pH was monitored using a pH meter with a glass electrode (Corning, pH 430). Small volumes of HCl (1 M) or NaOH (1 M) were added to the solution; the pH was measured, and UVvisible spectra were recorded. All spectra were adjusted for dilution. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||