Search and Browse
Download
Enter Data
|
Assay Method Information |
|
| | [3H]Serotonin Uptake Assay |
| Description: | The uptake assay was performed in reaction buffer containing 140 mM K2-tartrate, 10 mM Tricine, 10 mM Tris, and 5 mM MgCl2, pH 8.5. Liposomes (1 μl) were diluted into 200 μl of reaction buffer with 50 nM valinomycin and the indicated concentrations of the radiolabeled serotonin, usually 100 nM [3H]serotonin (PerkinElmer Life Sciences). Nonspecific accumulation of [3H]serotonin was measured in the presence of 5 μM reserpine or 15 μM nigericin and subtracted from the total transport. The reaction was stopped at the indicated time points by dilution of the mixture in 2 ml of ice-cold buffer and filtered on 0.22-μm GSWP (Millipore) filters. Radioactivity was measured using liquid scintillation. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||