Assay Method Information |
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| Colorimetric 5-LO Assay |
Description: | A colorimetric 5-LO assay kit (Lipoxygenase Inhibitor Screening Assay Kit) and potato 5-LO isolate were purchased from Cayman Chemicals (Ann Arbor, MI). 5-LO inhibition assay was performed according to the instruction manuals. All samples were dissolved in dimethyl sulfoxide, except for 1 and 2, which were dissolved in the assay buffers provided. All assays were performed in triplicate, and errors were calculated as standard deviation. |
Affinity data for this assay | |
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